Journal: STAR Protocols
Article Title: Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound screening
doi: 10.1016/j.xpro.2026.104393
Figure Lengend Snippet: tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs CD41/CD61) and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.
Article Snippet: Anti-human CD41/CD61 antibody (FITC); 1:50 , Miltenyi Biotec , CAT#130-124-887, RRID: AB_2819707.
Techniques: Marker, Selection, Generated